Monolithic columns coupled with distinctive affinity ligands offer you an unmatched Resolution for affinity purification of huge biomolecules.

The Resolute®️ AutoPak application was formulated in immediate response to this field need for packing consistency by thoroughly automated functions.

Sign up for our webinar to know what makes monoliths perfect for the issues of purifying huge biomolecules.

Lightweight, with a little footprint and simple upkeep, it’s frequently the cytometer of preference. It might be well suited for Individuals labs that involve…

This webpage doesn't exist as part of your chosen language. Your choice was saved and you may be notified once a web site may be considered with your language.

Guard columns: Guard columns are inserted just right before the particular column and capture something that might clog the true column. Rely on them!

I would love to join newsletters from Sartorius (Sartorius AG and its affiliated organizations) based of my private pursuits.

HPLC might have lower sensitivity for selected compounds, and a few cannot even be detected as They are really irreversibly adsorbed.

Effectiveness – supplied as the amount of theoretical plates, a critical metric for quantifying general performance of the separation    

Column treatment is exceptionally essential inside of a lab, on the other hand, Otherwise accomplished correctly can have an effect on your column’s wellbeing. check here Cleaning your HPLC column is likely get more info to be easy, but it is exceptionally imperative that you the efficiency, life time, and efficiency of the instrument.

By decreasing the pH of the solvent in a cation exchange column, As an illustration, extra hydrogen ions are offered to contend for positions over the anionic stationary phase, therefore eluting weakly bound cations.

Larger sized molecules are promptly washed in the column; lesser molecules penetrate the porous packing particles and elute afterwards.

Void volume is the level of Place inside a column that may be occupied by solvent. It is the Place throughout the column which is outside of the column's inner packing materials. Void volume is measured with a chromatogram as the primary component peak detected, which is frequently the solvent that was existing in the sample combination; Preferably the sample solvent flows throughout the column with out interacting With all the column, but is still detectable as distinct within the HPLC solvent. The void quantity is used like a correction issue.

Capillary columns (below 0.three mm) are applied almost solely with option detection signifies such as mass spectrometry. They are generally constructed from fused silica capillaries, in lieu of the stainless-steel tubing that larger sized columns hire.